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Old 02-05-2012, 10:44 AM   #11
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Join Date: Oct 2007
Location: Garcon Point
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Originally Posted by Carly View Post
Thanks for the replies everyone!
I'm actually researching how to collect enough DNA to create a giant mutant seatrout. Once I have my Frankentrout he can do all my lab work and I can go fishing.
My goal is to determine if there are genetically distinct seatrout populations in the Gulf. Since it's the most popular sport fish in Mississippi there is a stock enhancement program to supplement the wild population. Part of my project is to study the genetic diversity of the wild population to help enhance the stocking program.
Flounderpounder: Population genetics includes the study of genetic variation, gene flow among subpopulations and many other factors. Basically, it's the study of the genetic composition of biological populations.
Fin clippings are just small cuts from one of the fins, usually I take it from the dorsal or caudal fin. I only need a small part of fin to extract DNA from. I store them in sample tubes filled with DMSO which helps preserve the DNA. The scissors or knife, or whatever is used to take a piece of fin, needs to be rinsed and wiped off in between fish to avoid possible contamination. The best fin clips come from living fish but ones kept on ice also work well.
Hope this answers your questions.
I have a sampling protocol I will send to those who sent me a private message.
I really appreciate the help.

Interesting! Thanks for the info and good luck with your study! I hope when you are done, you'll give us a little update! If I wasn't between boats at the moment, I'd volunteer as well, but I'm sure the guys who already have will get what you need!
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speckled trout, specks, spotted seatrout



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